- Open Access
Trypanosoma rangeliTranscriptome Project: Generation and analysis of expressed sequence tags
© Snoeijer et al; licensee BioMed Central Ltd. 2004
- Received: 09 February 2004
- Accepted: 13 May 2004
- Published: 13 May 2004
Trypanosoma rangeli is an important hemoflagellate parasite of several mammalian species in Central and South America, sharing geographical areas, vectors and reservoirs with T. cruzi, the causative agent of Chagas disease. Thus, the occurrence of single and/or mixed infections, including in humans, must be expected and are of great importance for specific diagnosis and epidemiology. In comparison to several Trypanosomatidae species, the T. rangeli biology and genome are little known, reinforcing the needs of a gene discovery initiative. The T. rangeli transcriptome initiative aims to promote gene discovery through the generation of expressed sequence tags (ESTs) and Orestes (ORF ESTs) from both epimastigote and trypomastigote forms of the parasite, allowing further studies of the parasite biology, taxonomy and phylogeny.
- Taxonomic Position
- Vertebrate Host
- Trypomastigote Form
- Parasite Biology
Current status of the Trypanosoma rangeli transcriptome project.
EST matching with:
Number of matches*
Actual number of expressed sequence tags (ESTs) generated by the Trypanosoma rangeli transcriptome project distributed according to the similarity with sequences of distinct species.
Number of ESTs*
T. brucei brucei
T. brucei rhodesiense
Plasmodium yoelii yoelii
The number of studies involving T. rangeli is increasing due several aspects such as i) the importance on Chagas disease diagnosis and epidemiology, ii) the unknown life cycle on vertebrate hosts and iii) the contradictory taxonomic position of the parasite. Several molecular approaches have been used during the last few years to address these points. However, in order to perform comparative transcriptomics to address taxonomic studies, to bring up new markers for specific diagnosis, to discover, understand and/or compare some biological processes such as the involvement of the 3' end UTR on the post-transcriptional regulation of gene expression or even to assess intra-specific variability, a genomic panorama of the parasite must be revealed. Upon the release of the T. cruzi, T. brucei and L. major genome databases, not so far from now, the existence of well annotated ESTs and ORESTES from distinct T. rangeli forms will allow comparative transcriptomic studies with the state of the art technologies such as microarrays. Upon the end of the project, free accession to the T. rangeli transcriptome data, as well as tools for comparative genomics/transcriptomics under development, will be available through the UFSC Bioinformatics Laboratory website http://www.bioinformatica.ufsc.br.
The present work is part of C.Q. Snoeijer Masters thesis. All other authors have equally contributed to this work.
To the IBMP staff for their technical support on the cDNA libraries construction and to Dr. Ana Tereza R. de Vasconcellos from the Laboratório Nacional de Computação Científica (LNCC) for helping on bioinformatics analysis.
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