Primer | Position | Nucleic acid sequence | PCR product |
---|---|---|---|
External (outer primers) | |||
TE1 | 91–110 | (5)-AGGACGCAGAAATAGCAGTA (3) | 810-bp |
TE2 | 881–900 | (5)-ATTTAATTG AGTGGCGTGAG (3) |  |
Internal (nested Primers) | |||
TE3 | 311–330 | 5)-CTTTTATACGAGGAG GGGA (3) | 270-bp |
TE4 | 561–580 | 5)-TATGGGCGTGCAGATTTC AC (3) |  |
Semi-nested 1 | |||
TE1 | 91–110 | 5)-AGGACGCAGAAATAGCAGTA (3) | 490-bp |
TE4 | 561–580 | 5)-TATGGGCGTGCAGATTTC AC (3) |  |
Semi-nested 2 | |||
TE3 | 311–330 | 5)-CTTTTATACGAGGAG GGGA (3) | 590-bp |
TE2 | 881–900 | 5)-ATTTAATTG AGTGGCGTGAG (3) |  |