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Figure 5 | Kinetoplastid Biology and Disease

Figure 5

From: LmxMPK4, a mitogen-activated protein (MAP) kinase homologue essential for promastigotes and amastigotes of Leishmania mexicana

Figure 5

Deletion analysis of LmxMPK4. Southern blot analysis of different clones with a probe corresponding to the 3'-UTR of LmxMPK4. 1, L. mexicana wild type; 2, episome pXPACLmxMPK4 + ΔLmxMPK4+/- Phleo; 3, episome pXPACLmxMPK4 + ΔLmxMPK4+/- Hyg; 4, episome pXPACLmxMPK4 + ΔLmxMPK4-/-/+ Phleo/Hyg; 5, episome pXPACLmxMPK4 + ΔLmxMPK4-/- Phleo/Hyg. Numbers indicate the approximate size of DNA markers in kb.

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