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Figure 4 | Kinetoplastid Biology and Disease

Figure 4

From: LmxMPK4, a mitogen-activated protein (MAP) kinase homologue essential for promastigotes and amastigotes of Leishmania mexicana

Figure 4

Recombinant expression of LmxMPK4 and kinase assay. Left panels, Coomassie-stained gels; right panels, autoradiographs. A, recombinant GST-fusion proteins after kinase assay. Lanes 1 and 1', wild type LmxMPK4; lane 2 and 2', KM-mutant of LmxMPK4. B, kinase assay of GST-LmxMPK4 at different concentrations of Mg2+ and Mn2+. Lanes 1 and 1', 50 mM Mg2+; lanes 2 and 2', 10 mM Mg2+; lanes 3 and 3', 5 mM Mg2+; lanes 4 and 4', 10 mM Mg2+ and 2 mM Mn2+; lanes 5 and 5', 10 mM Mn2+; lanes 6 and 6', 2 mM Mn2+; lanes 7 and 7', 1 mM Mn2+. C, kinase assay of GST-LmxMPK4 at varying pH. Lanes 1 and 1', pH5.5; lanes 2 and 2', pH6.0; lanes 3 and 3', pH6.5; lanes 4 and 4', pH7.0; lanes 5 and 5', pH7.2; lanes 6 and 6', pH7.5; lanes 7 and 7', pH8.0; lanes 8 and 8', pH 8.5. Molecular masses of standard proteins are indicated in kilodalton.

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Kinetoplastid Biology and Disease

ISSN: 1475-9292