Figure 3From: LmxMPK4, a mitogen-activated protein (MAP) kinase homologue essential for promastigotes and amastigotes of Leishmania mexicanaImmunoblot of LmxMPK4 from L. mexicana wild type and episomal overexpressor. Lane 1, L. mexicana wild type promastigotes (4 × 107 cells); lane 2, amastigotes derived from mouse lesion (4 × 107 cells); lane 3, wild type in vitro differentiated amastigotes (1 × 108 cells); lane 4, promastigotes of LmxMPK4 episomal overexpressor (1 × 107 cells). A, blot probed with antiserum against the carboxy-terminal peptide of LmxMPK4; B, the blot was stripped and re-probed with a polyclonal antiserum against myo-inositol-1-phosphate synthase. The molecular masses of standard proteins are indicated in kilodaltons.Back to article page