Figure 4
From: Stage-specific expression of the mitochondrial co-chaperonin of Leishmania donovani, CPN10
(A) SDS-PAGE of various in vitro culture forms of L. donovani. Equivalent protein aliquots from L. donovani promastigotes, cultivated at either 25°C (promastigote -25°), at 37°C (promastigote -37°), and from axenic amastigotes after 3 days of in vitro differentiaton were subjected to Tricine Gel Electrophoresis and to SDS-PAGE. Protein bands were visualised by Coomassie Brilliant Blue staining. (B) Immunoblot analysis. A parallel gel was subjected to Western transfer and probed with an anti-CPN10 antibody. (C) Co-immune precipitation. Leishmania donovani lysate was precipitated using anti-CPN10 antibodies, separated by SDS-PAGE and subjected to WesternBlot analysis with anti-CPN60.2 antibodies. Lanes 1 and 2 are negative controls representing immune precipitations without anti-CPN10 antibody and with a preimmune antibody preparation, respectively. Lane 3 represents the CPN10 immune precipitation proper. The position of CPN60.2 is indicated.