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Figure 3 | Kinetoplastid Biology and Disease

Figure 3

From: Stage-specific expression of the mitochondrial co-chaperonin of Leishmania donovani, CPN10

Figure 3

Expression of L. donovani CPN10 in E. coli. The plasmids pJC45 or pCPN10-45 was used to transform E. coli strain BL21 (DE3) [pAPlacIQ]. Expression was induced by addition of IPTG, and the bacteria were harvested. After lysis, aliquots of the cultures before and after IPTG induction were analysed by SDS-PAGE and Coomassie Blue staining. The lysate containing the rCPN10 was subjected to metal chelate chromatography to purify the rCPN10 by virtue of its histidine tag. The purified rCPN10 was also subjected to SDS-PAGE analysis.

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