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Figure 5 | Kinetoplastid Biology and Disease

Figure 5

From: Experimental study of the function of the excreted/secreted LeishmaniaLmSIR2 protein by heterologous expression in eukaryotic cell line

Figure 5

Expression of a senescence biomarker. (pH6.0) β-galactosidase activity detected in L929 cells expressing LmSIR2 (Cl2) (B and C) but not in L929 carrying an empty pcDNA plasmid (A). Cells were seeded at 40 cells/mm2 in 25 cm2 flasks and after 13 days of growth, the presence of pH6-galactosidase activity (Arrow) was determined as described in the Materials and Methods section. N nucleus, note the perinuclear galactosidase activity.

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