Genetic subdivisions within Trypanosoma cruzi(Discrete Typing Units) and their relevance for molecular epidemiology and experimental evolution

Table 1 Criteria and tests of clonality (after 10)

Criterion	Description
Segregation (within locus)
a	Fixed heterozygosity *
b	Absence of segregation genotypes *
c	Deviation from Hardy-Weinberg expectation
Recombination (beween loci)
d	Overrepresented, identical genotypes widespread * (statistical tests d1 # and d2 §
e	Absence of recombinant genotypes ** §/P>
f	Linkage disequilibrium ** §/P>
g	Correlation between two independent sets of genetic markers ** §P

* = used qualitatively, without statistical calculations; ** = used with statistical calculations. # Combinatorial analysis §Monte Carlo simulation with 104 iterations ¶Mantel nonparametric test of correlation between genetic distances obtained from different markers