Figure 1

A. Neighbor-joining tree based on GAPDH nucleic-acid sequences. The horizontal bar represents five substitutions per 100 nucleotides. Numbers at the nodes are bootstrap values (1000 samplings). Accession numbers of sequences used in the analysis: AF322391, Blastocrithidia gerricola; AF047493, Crithidia fasciculata; AF053739, Crithidia fasciculata 2; AF053740, Crithidia luciliae; L39772, Euglena gracilis; AF047494, Herpetomonas samuelpessoai; AF047497, Leishmania major; X65226, Leishmania mexicana; AF053741, Leptomonas lactosovorans; AF322390, Leptomonas peterhoffi; AY029072, Leptomonas pyrrhocoris; AF047495, Leptomonas seymouri; AF320820, Leptomonas sp. Cfm; AF339451, Leptomonas sp. Nfm; AF375664, Leptomonas sp. F2; AF047496, Phytomonas sp.; AF047499, Trypanosoma brucei gambiense; AF047498, Trypanosoma congolense; X52898, Trypanosoma cruzi; AF053743, Trypanosoma evansi; AF047500, Trypanosoma vivax; AF053742, Trypanosoma rangeli; AF053744, Trypanosoma vivax; AF316620, Wallaceina brevicula; X74535, Trypanoplasma borreli. B. Phylogenetic tree of eukaryotic aldolases. The tree was created using both neighbor-joining and maximum likelihood methods, with and without gamma correction for unequal rates of evolution of sites. Neigbor-joining analysis was able to divide the sequences into three major clades: chloroplast sequences, plant cytosolic sequences and aldolases of the remainder of the eukaryotes. The trypanosomatids clustered witht the chloroplasts sequences, while the chloroplast sequence from Euglena and those of Plasmodium grouped with the other non-plant aldolases. However, there was no bootstrap support for any of the deep-branching groups. Maximum likelihood analyses grouped all plant sequences (chloroplast and cytosol) as well as the sequences from Euglena, Plasmodium and the Trypanosomatidae together in a single star-like subtree.